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If the only time you had sexual exposure all September was the 25th, and a normal period came as expected before the end of the month, you are not pregnant. The egg comes 2 weeks before your period and is only good for a day. If 1) you were fooling around two weeks before your period and some semen accidentally got in you, and if 2)your period was unusually light, and if 3) you are experiencing some early pregnancy signs, then yes, you should take a test any day now using first morning pee. GL!