Hey, I was looking up the Hook Effect and found this on an ICON 25hCG pregnancy test site. The information was interesting and may effect some of your test results.
Q: Is there any “hook” effect? If so, at what level of hCG? If not, what is the highest level of hCG tested?
A: The product provides the correct result up to 500,000 mIU hCG/mL.
Q: What part of the molecule is detected?
A: The product detects intact hCG.
Q: When is the best time of day to obtain a urine specimen for testing?
A: Urine specimens collected at any time of day may be used on ICON® 25 hCG tests. However, a first morning urine specimen is preferred if possible as it usually contains the highest concentration of hCG.
Q: If the test is used prior to the equilibration of the specimen and test to room temperature, is the result reliable?
A: No. The test and the specimen must equilibrate to room temperature (15 to 30° C) prior to testing.
Q: If too much or not enough of the specimen sample was added, is the result still reliable?
A: No, an incorrect amount of specimen sample can affect the test result. It should be repeated with a new test and the appropriate amount of sample.
These last 2 questions and answers really interested me. I don't know that we all have let our specimin and our test come to the same temp. before testing. Could be a factor, hmmm?
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